DEACYLATION OF BENZYLPENICILLIN BY IMMOBILIZED PENICILLIN ACYLASE IN A CONTINUOUS 4-STAGE STIRRED-TANK REACTOR

被引：28

作者：

CARLEYSMITH, SW ^{[1
]}

LILLY, MD ^{[1
]}

机构：

[1] UNIV LONDON UNIV COLL, DEPT CHEM & BIOCHEM ENGN, LONDON WC1E 7JE, ENGLAND

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 1979年 / 21卷 / 06期

关键词：

D O I：

10.1002/bit.260210610

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Immobilized penicillin acylase [EC 3.5.1.11 from Escherichia coli] was used for the deacylation of benzylpenicillin at 37.degree. C in a continuous reactor consisting of four 1 l stirred tanks connected in series. There was good agreement between the predicted and actual conversions obtained in each tank under steady-state conditions. The operational stability of the immobilized enzyme in the tanks depended on the pH and the rate of addition and concentration of alkali needed to neutralize the acid produced during the reaction. At pH 7 with the addition of 2 M NaOH, the half-life for enzyme stability was greater than 400 h in all tanks. This was over half the value for the immobilized enzyme when stored at 37.degree. C and pH 7.

引用

页码：1057 / 1073

页数：17

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