A NOVEL METHOD OF PREPARING RAT-MONOCLONAL ANTIBODY-PRODUCING HYBRIDOMAS BY USING RAT MEDIAL ILIAC LYMPH-NODE CELLS

被引：168
作者：
KISHIRO, Y
KAGAWA, M
NAITO, I
SADO, Y
机构：
[1] SHIGA MED RES INST, DIV IMMUNOL, OKAYAMA, JAPAN
[2] SHIGA MED RES INST, DIV ULTRASTRUCT RES, OKAYAMA, JAPAN
来源：
CELL STRUCTURE AND FUNCTION | 1995年 / 20卷 / 02期
关键词：
RAT MONOCLONAL ANTIBODY; MEDIAL ILIAC LYMPH NODE CELLS; RAT MOUSE HYBRIDOMAS; CELL FUSION;
D O I：
10.1247/csf.20.151
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
A novel method of preparing hybridomas producing rat monoclonal antibodies was established. The enlarged medial iliac lymph nodes from rats injected via hind footpads with an emulsion of antigen and Freund's adjuvant were used for cell fusion. Ovalbumin was used as a representative antigen. The incidence of hybridomas producing antibody of interest with this method was about 10 times higher than that of hybridomas with the conventional method using mouse or rat spleen cells. The average percentages of hybridomas producing IgG1, IgG2a, IgG2b and IgG2c were 37.1%, 47.0%, 15.9% and 0.0%, respectively. A single injection with antigen was sufficient for immunization in this method.
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收藏
页码：151 / 156
页数：6
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