DISRUPTION OF THE GOLGI-APPARATUS BY BREFELDIN-A BLOCKS CELL POLARIZATION AND INHIBITS DIRECTED CELL-MIGRATION

被引：105

作者：

BERSHADSKY, AD ^{[1
]}

FUTERMAN, AH ^{[1
]}

机构：

[1] WEIZMANN INST SCI, DEPT MEMBRANE RES & BIOPHYS, IL-76100 REHOVOT, ISRAEL

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1994年 / 91卷 / 12期

关键词：

D O I：

10.1073/pnas.91.12.5686

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The role of the Golgi apparatus in the motile activity of fibroblasts was examined with brefeldin A (BFA), which disrupts the Golgi apparatus in a variety of cells. Upon incubation with BFA, Swiss mouse 3T3 fibroblasts lost their typical polarized morphology, in which the leading edge is characterized by intensive lamellipodia formation. BFA affected cell asymmetry as demonstrated by a decrease in the morphometric indices, dispersion, and elongation. After BFA treatment, cells showed little protrusional activity and did not form a dense actin network at the leading edge, and consequently the rate of cell migration into an experimental wound was significantly reduced. In addition, BFA prevented an increase in pseudopodial activity and prevented the formation of long processes induced by phorbol 12-myristate 13-acetate. The effects of BFA on cell shape and protrusional activity were quantitatively similar to those observed with the microtubule-disrupting agent nocodazole, although BFA had no effect on microtubule integrity. These results suggest that the integrity of both the Golgi apparatus and microtubules is necessary for the generation and maintenance of fibroblast asymmetry, which is a prerequisite for directed cell migration.

引用

页码：5686 / 5689

页数：4

共 30 条

[1] MEMBRANE INSERTION AT THE LEADING-EDGE OF MOTILE FIBROBLASTS [J].

BERGMANN, JE ;

KUPFER, A ;

SINGER, SJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (05) :1367-1371

[2]

BERSHADSKY AD, 1993, SYM SOC EXP BIOL, V47, P353

[3] PSEUDOPODIAL ACTIVITY AT THE ACTIVE EDGE OF MIGRATING FIBROBLAST IS DECREASED AFTER DRUG-INDUCED MICROTUBULE DEPOLYMERIZATION [J].

BERSHADSKY, AD ;

VAISBERG, EA ;

VASILIEV, JM .

CELL MOTILITY AND THE CYTOSKELETON, 1991, 19 (03) :152-158

[4]

Bray D., 1992, CELL MOVEMENTS

[5]

BRAY RL, 1992, NEW YORK TIMES BK R, P7

[6] DISRUPTION OF THE GOLGI-APPARATUS WITH BREFELDIN-A DOES NOT DESTABILIZE THE ASSOCIATED DETYROSINATED MICROTUBULE NETWORK [J].

BURGESS, TL ;

SKOUFIAS, DA ;

WILSON, L .

CELL MOTILITY AND THE CYTOSKELETON, 1991, 20 (04) :289-300

[7] BREFELDIN-A INHIBITS GOLGI MEMBRANE-CATALYZED EXCHANGE OF GUANINE-NUCLEOTIDE ONTO ARF PROTEIN [J].

DONALDSON, JG ;

FINAZZI, D ;

KLAUSNER, RD .

NATURE, 1992, 360 (6402) :350-352

[8] SPECIAL TYPE OF MORPHOLOGICAL REORGANIZATION INDUCED BY PHORBOL ESTER - REVERSIBLE PARTITION OF CELL INTO MOTILE AND STABLE DOMAINS [J].

DUGINA, VB ;

SVITKINA, TM ;

VASILIEV, JM ;

GELFAND, IM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (12) :4122-4125

[9]

DUNN GA, 1986, J CELL SCI, V83, P313

[10] SUPPRESSION OF KINESIN EXPRESSION IN CULTURED HIPPOCAMPAL-NEURONS USING ANTISENSE OLIGONUCLEOTIDES [J].

FERREIRA, A ;

NICLAS, J ;

VALE, RD ;

BANKER, G ;

KOSIK, KS .

JOURNAL OF CELL BIOLOGY, 1992, 117 (03) :595-606

← 1 2 3 →