ISOLATION AND STRUCTURE OF A BRAIN CONSTITUENT THAT BINDS TO THE CANNABINOID RECEPTOR

被引：4400
作者：
DEVANE, WA
HANUS, L
BREUER, A
PERTWEE, RG
STEVENSON, LA
GRIFFIN, G
GIBSON, D
MANDELBAUM, A
ETINGER, A
MECHOULAM, R
机构：
[1] HEBREW UNIV JERUSALEM,FAC MED,DEPT NAT PROD,IL-91120 JERUSALEM,ISRAEL
[2] UNIV ABERDEEN MARISCHAL COLL,DEPT BIOMED SCI,ABERDEEN AB9 1AS,SCOTLAND
[3] TECHNION ISRAEL INST TECHNOL,DEPT CHEM,IL-32000 HAIFA,ISRAEL
来源：
SCIENCE | 1992年 / 258卷 / 5090期
基金：
英国惠康基金;
关键词：
D O I：
10.1126/science.1470919
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Arachidonylethanolamide, an arachidonic acid derivative in porcine brain, was identified in a screen for endogenous ligands for the cannabinoid receptor. The structure of this compound, which has been named "anandamide," was determined by mass spectrometry and nuclear magnetic resonance spectroscopy and was confirmed by synthesis. Anandamide inhibited the specific binding of a radiolabeled cannabinoid probe to synaptosomal membranes in a manner typical of competitive ligands and produced a concentration-dependent inhibition of the electrically evoked twitch response of the mouse vas deferens, a characteristic effect of psychotropic cannabinoids. These properties suggest that anandamide may function as a natural ligand for the cannabinoid receptor.
引用
收藏
页码：1946 / 1949
页数：4
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