ANALYSIS OF ELECTROPHORETIC WHOLE-CELL PROTEIN PROFILES AS A TOOL FOR CHARACTERIZATION OF ENTEROCOCCUS SPECIES

被引：49
作者：
MERQUIOR, VLC
PERALTA, JM
FACKLAM, RR
TEIXEIRA, LM
机构：
[1] UNIV FED RIO DE JANEIRO,INST MICROBIOL,BR-21941 RIO JANEIRO,BRAZIL
[2] CTR DIS CONTROL & PREVENT,RESP DIS BRANCH,ATLANTA,GA
来源：
CURRENT MICROBIOLOGY | 1994年 / 28卷 / 03期
关键词：
D O I：
10.1007/BF01571056
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The whole-cell protein-profiling technique was evaluated for identifying Enterococcus species. Reference strains, strains from human infections, from animals other than human, and from environmental sources were studied. Whole-cell extracts were obtained by lysozyme treatment and were analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis and densitometry. Each Enterococcus species had a unique and distinguishable whole-cell protein profile. The major differences among species-specific profiles were found in the positions corresponding to 60-40 and 30-20 kDa. Profiles of the same species did not show qualitative variations. Analysis of whole-cell protein profiles was shown to be a relatively simple, easy, and reproducible procedure for the reliable and fast differentiation and identification of the enterococcal species.
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页码：149 / 153
页数：5
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