CHARACTERIZATION AND PARTIAL-PURIFICATION OF A MEMBRANE-PROTEIN FROM RABBIT SKELETAL-MUSCLE WHICH INHIBITS THE CAMP-DEPENDENT PROTEIN-KINASE

被引：1

作者：

WOSCHOLSKI, R ^{[1
]}

MARME, D ^{[1
]}

机构：

[1] GODECKE AG,UNIV FREIBURG,INST MOLEC CELL BIOL,W-7800 FREIBURG,GERMANY

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1992年 / 182卷 / 01期

关键词：

D O I：

10.1016/S0006-291X(05)80135-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Rabbit skeletal muscle membranes contain a protein which inhibits the cAMP-dependent protein kinase. The activity of the partially purified membrane protein is characterized by an IC-50 of 10 to 30 nM with respect to the inhibition of the activity of the catalytic subunit of the cAMP-dependent protein kinase and is sensitive to treatment with heat, acid, alkali and trypsin. The active fractions contain proteins ranging from 40 to 120 kDa, analysed by SDS-gel electrophoresis. © 1992 Academic Press, Inc.

引用

页码：232 / 237

页数：6

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