PHOTOSYNTHESIS;
REACTION CENTER;
GENETIC MODIFICATION;
RESONANCE RAMAN SPECTRUM;
ELECTRON TRANSFER;
PIGMENT PROTEIN INTERACTION;
D O I:
10.1016/S0005-2728(05)80076-3
中图分类号:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号:
071010 ;
081704 ;
摘要:
Resonance Raman spectra are reported for RCs from Rb. capsulatus in which the L104 glutamic acid is replaced by glutamine. The skeletal modes of the primary electron acceptor, BPh(L), in these RCs undergo temperature-dependent frequency shifts that are identical to those observed for BPh(L) in RCs from wild-type. This observation suggests that the strength of the hydrogen bond between the L104 residue and the C9 keto group of BPh(L) is not a determinant of the temperature-dependent conformation of this pigment.