ACYL COENZYME-A-6-AMINOPENICILLANIC ACID ACYLTRANSFERASE FROM PENICILLIUM-CHRYSOGENUM AND ASPERGILLUS-NIDULANS

被引：54
|
作者：
WHITEMAN, PA
ABRAHAM, EP
BALDWIN, JE
FLEMING, MD
SCHOFIELD, CJ
SUTHERLAND, JD
WILLIS, AC
机构：
[1] MRC,IMMUNOCHEM UNIT,DEPT BIOCHEM,OXFORD OX1 3QU,ENGLAND
[2] DYSON PERRINS LAB,OXFORD OX1 3QY,ENGLAND
[3] UNIV OXFORD,CTR MOLEC BIOL,OXFORD OX1 3QY,ENGLAND
来源：
FEBS LETTERS | 1990年 / 262卷 / 02期
关键词：
Acyltransferase; Aspergillus; Benzylpenicillin; Isopenicillin N; Penicillin biosynthesis; Penicillium;
D O I：
10.1016/0014-5793(90)80224-7
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A study of the final stages of the biosynthesis of the penicillins in Penicillium chrysogenum has revealed two types of enzyme. One hydrolyses phenoxymethyl penicillin to 6-aminopenicillanic acid (6-APA). The other, also obtained from Aspergillus nidulans, transfers a phenylacetyl group from phenylacetyl CoA to 6-APA. The acyltransferase, purified to apparent homogeneity, had a molecular mass of 40 kDa. It also catalyses the conversion of isopenicillin N (IPN) to benzylpenicillin (Pen G) and hydrolyses IPN to 6-APA. In the presence of SDS it dissociates, with loss of activity, into fragments of ca 30 and 10.5 kDa, but activity is regained when these fragments recombine in the absence of SDS. © 1990.
引用
收藏
页码：342 / 344
页数：3
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