CLONING AND CHARACTERIZATION OF TRYPSIN-INHIBITOR GENE FROM VIGNA-UNGUICULATA

被引：3

作者：

BIJOLA, V ^{[1
]}

PATHRE, U ^{[1
]}

NATH, P ^{[1
]}

SANE, PV ^{[1
]}

机构：

[1] NATL BOT RES INST,LUCKNOW 226001,UTTAR PRADESH,INDIA

来源：

JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY | 1994年 / 3卷 / 02期

关键词：

COWPEA TRYPSIN INHIBITOR; PURIFICATION; AMPLIFICATION; PROTEIN EXPRESSION; SEQUENCING;

D O I：

10.1007/BF03321961

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A trypsin inhibitor, member of Bowman-Birk family, was isolated and purified from cowpea (Vigna unguiculata). Polyclonal antibodies were raised against cowpea trypsin inhibitor (CpTI) protein in rabbits. The gene for CpTI was amplified by polymerase chain reaction and cloned in a bacterial expression vector pVCATFR18416. The expression of CpTI protein in BL21 (DE3) strain of Escherichia coli. was confirmed by western blot studies. The CpTI gene was also sequenced and found to exhibit 100% homology with already published sequence of CpTI gene.

引用

页码：113 / 119

页数：7

共 27 条

[1] BASU B, 1991, INDIAN J EXP BIOL, V29, P1002
[2] BIRK Y, 1985, INT J PEPT PROT RES, V25, P112
[3] BURNETTE WN, 1981, ANAL BIOCHEM, V112, P195, DOI 10.1016/0003-2697(81)90281-5
[4] CHAENG J, 1992, PLANT SCI, V8, P83
[5] CHARACTERISTICS OF MICROPLATE METHOD OF ENZYME-LINKED IMMUNOSORBENT ASSAY FOR DETECTION OF PLANT-VIRUSES
CLARK, MF
ADAMS, AN
[J]. JOURNAL OF GENERAL VIROLOGY, 1977, 34 (MAR) : 475 - 483
[6] Dellaporta S.L., 1983, PLANT MOL BIOL REP, V1, P19, DOI [10.1007/BF02712670, DOI 10.1007/BF02712670]
[7] PREPARATION AND PROPERTIES OF 2 NEW CHROMOGENIC SUBSTRATES OF TRYPSIN
ERLANGER, BF
COHEN, W
KOKOWSKY, N
[J]. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1961, 95 (02) : 271 - &
[8] INSECT TOLERANT TRANSGENIC TOMATO PLANTS
FISCHHOFF, DA
BOWDISH, KS
PERLAK, FJ
MARRONE, PG
MCCORMICK, SM
NIEDERMEYER, JG
DEAN, DA
KUSANOKRETZMER, K
MAYER, EJ
ROCHESTER, DE
ROGERS, SG
FRALEY, RT
[J]. BIO-TECHNOLOGY, 1987, 5 (08): : 807 - 813
[9] FOARD D E, 1982, Plant Molecular Biology, V1, P227, DOI 10.1007/BF00021034
[10] FUJIMOTO H, 1993, BIO-TECHNOL, V11, P1151, DOI 10.1038/nbt1093-1151

← 1 2 3 →