CHARACTERIZATION OF A C-ALPHA GENE OF SWINE

被引:32
作者
BROWN, WR
BUTLER, JE
机构
[1] Department of Microbiology, University of Iowa Medical School, Iowa City
关键词
IGA; SWINE; HEAVY CHAIN; CDNA; NUCLEOTIDE SEQUENCE;
D O I
10.1016/0161-5890(94)90171-6
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The cDNA sequence encoding the constant region of the porcine IgA heavy chain as well as the exon-intron structure of the germline gene, have been determined. A cDNA clone (1A1) spanning the CH3 domain and part of the CH2 domain was isolated from a porcine mesenteric lymph node cDNA library. Clone 1A1 was aligned with a PCR-generated DNA fragment encompassing the CH1 domain through the 5' end of the CH3 domain to derive the complete cDNA sequence. Comparison with other mammalian C alpha heavy chains (hinge regions excluded) indicated that the deduced amino acid sequence of porcine C alpha is most homologous with the human C alpha subclasses (> 70%), followed by mouse C alpha (61%) and a consensus sequence of the 13 rabbit C alpha heavy chains (59%). The greatest sequence homology was found among the CH3 domains in all species. A striking feature of porcine C alpha is its short six amino acid hinge which like other mammalian IgAs, is encoded with the CH2 domain. Sequence analysis of germline C alpha, generated by PCR from liver or sperm DNA, revealed an exon-intron organization similar to other mammalian C alpha genes. Genomic Southern blot data are consistent with the presence of a single C alpha gene within the porcine genome. Data obtained in these studies will be valuable in pursuing the use of swine as a model in immunological research.
引用
收藏
页码:633 / 642
页数:10
相关论文
共 50 条
[1]   BASIC LOCAL ALIGNMENT SEARCH TOOL [J].
ALTSCHUL, SF ;
GISH, W ;
MILLER, W ;
MYERS, EW ;
LIPMAN, DJ .
JOURNAL OF MOLECULAR BIOLOGY, 1990, 215 (03) :403-410
[2]   MOUSE IMMUNOGLOBULIN-A - NUCLEOTIDE-SEQUENCE OF THE STRUCTURAL GENE FOR THE ALPHA-HEAVY CHAIN DERIVED FROM CLONED CDNAS [J].
AUFFRAY, C ;
NAGEOTTE, R ;
SIKORAV, JL ;
HEIDMANN, O ;
ROUGEON, F .
GENE, 1981, 13 (04) :365-374
[3]   ANTIBODY-RESPONSES IN SERUM, COLOSTRUM, AND MILK OF SWINE AFTER INFECTION OR VACCINATION WITH TRANSMISSIBLE GASTROENTERITIS VIRUS [J].
BOHL, EH ;
SAIF, LJ ;
OLQUIN, MVF ;
GUPTA, RKP .
INFECTION AND IMMUNITY, 1972, 6 (03) :289-+
[4]   CHARACTERIZATION OF A CDNA CLONE ENCODING FOR A PORCINE IMMUNOGLOBULIN MU-CHAIN [J].
BOSCH, BL ;
BEAMAN, KD ;
KIM, YB .
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY, 1992, 16 (04) :329-337
[5]  
BOURNE FJ, 1973, IMMUNOLOGY, V24, P157
[6]   IGA IMMUNOGLOBULIN FROM PORCINE SERUM [J].
BOURNE, FJ .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1969, 36 (01) :138-&
[7]   OVALBUMIN GENE - EVIDENCE FOR A LEADER SEQUENCE IN MESSENGER-RNA AND DNA SEQUENCES AT EXON-INTRON BOUNDARIES [J].
BREATHNACH, R ;
BENOIST, C ;
OHARE, K ;
GANNON, F ;
CHAMBON, P .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1978, 75 (10) :4853-4857
[8]   THE IGA HEAVY-CHAIN GENE FAMILY IN RABBIT - CLONING AND SEQUENCE-ANALYSIS OF 13 C-ALPHA-GENES [J].
BURNETT, RC ;
HANLY, WC ;
ZHAI, SK ;
KNIGHT, KL .
EMBO JOURNAL, 1989, 8 (13) :4041-4047
[9]  
BUTLER J E, 1974, P217
[10]  
BUTLER JE, 1979, SEMIN PERINATOL, V3, P255