INHERITANCE OF KANAMYCIN AND ETHIONINE RESISTANCE INTRODUCED IN ALFALFA (MEDICAGO-SATIVA L) BY GENE-TRANSFER AND CELL SELECTION

被引：1

作者：

SARUL, P

VLAHOVA, M

TODOROVSKA, E

ATANASSOV, A

机构：

[1] INST GENET ENGN, KOSTINBROD, BULGARIA

[2] INST FORAGE CROPS, ULAANBAATAR, MONGOLIA

[3] DE MONTFORD UNIV, NORMAN BORLAUG CTR PLANT SCI RES, KOSTINBROD, BULGARIA

来源：

BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT | 1995年 / 9卷 / 01期

关键词：

D O I：

10.1080/13102818.1995.10818821

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Fertile kanamycin resistant plants of Medicago sativa L. were obtained by genetic transformation with binary vector of Agrobacterium tumefaciens and were used as a fertilizer of L-ethionine (ETH) resistant alfalfa variants. The dominant nature of both kanamycin and L-ethionine resistant traits was demonstrated by the double resistant phenotype of obtained hybrid plants. Southern blot analysis showed that transgenic plants and self-pollinated progeny harbored the kanamycin gene, and expressed the neomycin phosphotransferase (npt) activity in vitro. The inheritance of both single (kanamycin) and double (kanamycin and L-ethionine) resistance traits was analyzed and the way in which marker characters are segregated is discussed.

引用

页码：45 / 51

页数：7

共 40 条

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