CYTOLOGIC DETECTION OF HERPES-SIMPLEX VIRUS-DNA IN NIPPLE DISCHARGE BY INSITU HYBRIDIZATION - REPORT OF 2 CASES

被引：7
作者：
KOBAYASHI, TK
OKAMOTO, H
YAKUSHIJI, M
机构：
[1] Department of Cytopathology, Saiseikai Shiga Hospital, Imperial Gift Foundation, Inc, Shiga and Department of Obstetrics and Gynecology, Kurume University Medical School, Fukuoka
来源：
DIAGNOSTIC CYTOPATHOLOGY | 1993年 / 9卷 / 03期
关键词：
HSV DNA; CYTOLOGY; INSITU HYBRIDIZATION; NIPPLE SECRETION; BREAST MILK;
D O I：
10.1002/dc.2840090310
中图分类号：
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号：
1001 ;
摘要：
Cytologic changes in the smears from nipple discharge of two cases with herpes simplex virus (HSV) infections are described The cytology revealed the ground-glass appearance of the nuclei with multinucleated syncytial cells. Subsequently, in situ hybridization (ISH) using a biotinylated DNA probe was applied to identify the HSV DNA in the Papanicolaou-destained specimens. Positive hybridization was found with intense staining for the HSV DNA in the nuclei of cells having a ground-glass appearance. Cytologic observation together with ISH procedure may be rapid and valuable tool for the detection and final demonstration of HSV infections. Similar investigations may be carried out in cellular samples from other body sites.
引用
收藏
页码：296 / 299
页数：4
相关论文
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