SEROLOGICAL RESPONSE IN LEPROSY AND TUBERCULOSIS PATIENTS TO THE 18-KDA ANTIGEN OF MYCOBACTERIUM-LEPRAE AND ANTIGEN 85B OF MYCOBACTERIUM-BOVIS BCG

被引：0
|
作者：
VIKERFORS, T
OLCEN, P
WIKER, H
WATSON, JD
机构：
[1] OREBRO MED CTR HOSP, DEPT CLIN MICROBIOL & IMMUNOL, S-70185 OREBRO, SWEDEN
[2] UNIV OSLO, INST IMMUNOL & RHEUMATOL, OSLO, NORWAY
[3] UNIV AUCKLAND, DEPT MOLEC MED, AUCKLAND, NEW ZEALAND
来源：
INTERNATIONAL JOURNAL OF LEPROSY AND OTHER MYCOBACTERIAL DISEASES | 1993年 / 61卷 / 04期
关键词：
D O I：
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中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Two enzyme-linked immunosorbent assays (ELISAs) for the detection of leprosy antibodies were developed. These assays were based on the recombinant 18-kDa protein of Mycobacterium leprae and the 85B antigen of M. bovis. Sera from leprosy and tuberculosis patients were analyzed, and a phenolic glycolipid-I (PGL-I) ELISA was used as a reference test. The 18-kDa ELISA reached a sensitivity of 70% on lepromatous leprosy sera. The corresponding results for the 85B and the PGL-I ELISAs were 93% and 96%, respectively. The sensitivity on tuberculoid leprosy sera was 72% for the 18-kDa antigen, 95% for the 85B antigen, and 60% for the PGL-I antigen. The 18-kDa antigen ELISA was found to have crossreactivity to sera from patients with tuberculosis, while the 85B and PGL-I assays had high specificity.
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页码：571 / 580
页数：10
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