A SHORT FORM OF THE TICK-BORNE ENCEPHALITIS-VIRUS NS3 PROTEIN

被引：14
作者：
PUGACHEV, KV [1 ]
NOMOKONOVA, NY [1 ]
MOROZOVA, OV [1 ]
PLETNEV, AG [1 ]
机构：
[1] ACAD MED SCI USSR,INST THERAPY,NOVOSIBIRSK 630003,USSR
来源：
FEBS LETTERS | 1992年 / 297卷 / 1-2期
关键词：
FLAVIVIRUS; NONSTRUCTURAL PROTEIN; CLEAVAGE;
D O I：
10.1016/0014-5793(92)80329-F
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Using monoclonal antibodies to the tick-borne encephalitis virus (TBE) nonstructural protein NS3 two forms of this protein were revealed in TBE-infected mammalian cells: a full-length form (69 kDa) and a short form (49 kDa) which has not been observed before and was called NS3'. Recombinant plasmids were constructed and various fragments of the TBE NS3 gene were expressed in rabbit reticulocyte lysate. By analyzing immune precipitates of S-35-labeled translation products, we could monitor and localize internal cleavage of NS3, due to which the NS3' protein was generated.
引用
收藏
页码：67 / 69
页数：3
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