RECOMBINANT HUMAN BONE MORPHOGENETIC PROTEIN INDUCES BONE-FORMATION

被引：1315
作者：
WANG, EA
ROSEN, V
DALESSANDRO, JS
BAUDUY, M
CORDES, P
HARADA, T
ISRAEL, DI
HEWICK, RM
KERNS, KM
LAPAN, P
LUXENBERG, DP
MCQUAID, D
MOUTSATSOS, IK
NOVE, J
WOZNEY, JM
机构：
[1] Genetics Institute, Cambridge, MA 02140
来源：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1990年 / 87卷 / 06期
关键词：
cartilage induction;
D O I：
10.1073/pnas.87.6.2220
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
We have purified and characterized active recombinant human bone morphogenetic protein (BMP) 2A. Implantation of the recombinant protein in rats showed that a single BMP can induce bone formation in vivo. A dose-response and time-course study using the rat ectopic bone formation assay revealed that implantation of 0.5-115 μg of partially purified recombinant human BMP-2A resulted in cartilage by day 7 and bone formation by day 14. The time at which bone formation occurred was dependent on the amount of BMP-2A implanted; at high doses bone formation could be observed at 5 days. The cartilage- and bone-inductive activity of the recombinant BMP-2A is histologically indistinguishable from that of bone extracts. Thus, recombinant BMP-2A has therapeutic potential to promote de novo bone formation in humans.
引用
收藏
页码：2220 / 2224
页数：5
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