RECOMBINANT HUMAN BONE MORPHOGENETIC PROTEIN INDUCES BONE-FORMATION

被引：1323

作者：

WANG, EA

ROSEN, V

DALESSANDRO, JS

BAUDUY, M

CORDES, P

HARADA, T

ISRAEL, DI

HEWICK, RM

KERNS, KM

LAPAN, P

LUXENBERG, DP

MCQUAID, D

MOUTSATSOS, IK

NOVE, J

WOZNEY, JM

机构：

[1] Genetics Institute, Cambridge, MA 02140

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1990年 / 87卷 / 06期

关键词：

cartilage induction;

D O I：

10.1073/pnas.87.6.2220

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We have purified and characterized active recombinant human bone morphogenetic protein (BMP) 2A. Implantation of the recombinant protein in rats showed that a single BMP can induce bone formation in vivo. A dose-response and time-course study using the rat ectopic bone formation assay revealed that implantation of 0.5-115 μg of partially purified recombinant human BMP-2A resulted in cartilage by day 7 and bone formation by day 14. The time at which bone formation occurred was dependent on the amount of BMP-2A implanted; at high doses bone formation could be observed at 5 days. The cartilage- and bone-inductive activity of the recombinant BMP-2A is histologically indistinguishable from that of bone extracts. Thus, recombinant BMP-2A has therapeutic potential to promote de novo bone formation in humans.

引用

页码：2220 / 2224

页数：5

共 23 条

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