TRANSCRIPTION OF THE PROMOTER OF THE RAT NF-1 GENE DEPENDS ON THE INTEGRITY OF AN SP1 RECOGNITION SITE

被引：22

作者：

AMMENDOLA, R ^{[1
]}

GOUNARI, F ^{[1
]}

PIAGGIO, G ^{[1
]}

DESIMONE, V ^{[1
]}

CORTESE, R ^{[1
]}

机构：

[1] EUROPEAN MOLEC BIOL LAB,MEYERHOFSTR 1,W-6900 HEIDELBERG,GERMANY

来源：

MOLECULAR AND CELLULAR BIOLOGY | 1990年 / 10卷 / 01期

关键词：

D O I：

10.1128/MCB.10.1.387

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The transcription start site and promoter of the rat gene coding for the transcription factor NF-1 have been identified. The NF-1 promoter was fused to the chloramphenicol acetyltransferase-coding sequence, and the resulting plasmid was transcriptionally active in the HepG2 cell line. Footprinting and gel retardation analysis indicated that the transcription factor Sp1 binds to the NF-1 promoter. Mutants in the Sp1-binding site displayed a strong reduction in transcriptional activity.

引用

页码：387 / 390

页数：4

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