GENETIC-TRANSFORMATION OF BANANA AND PLANTAIN (MUSA SPP) VIA PARTICLE BOMBARDMENT

被引：140
|
作者：
SAGI, L [1 ]
PANIS, B [1 ]
REMY, S [1 ]
SCHOOFS, H [1 ]
DESMET, K [1 ]
SWENNEN, R [1 ]
CAMMUE, BPA [1 ]
机构：
[1] CATHOLIC UNIV LEUVEN,FA JANSSENS LAB GENET,B-3001 LOUVAIN,BELGIUM
来源：
BIO-TECHNOLOGY | 1995年 / 13卷 / 05期
关键词：
D O I：
10.1038/nbt0595-481
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
We have developed a simple protocol to allow the production of transgenic banana plants. Foreign genes were delivered into embryogenic suspension cells using accelerated particles coated with DNA. Bombardment parameters were optimized for a modified particle gun resulting in high levels of transient expression of the beta-glucuronidase gene in both banana and plantain cells. Bombarded banana cells were selected with hygromycin and regenerated into plants. Molecular and histochemical characterization of transformants revealed the stable integration of the transferred genes into the banana genome.
引用
收藏
页码：481 / 485
页数：5
相关论文
共 50 条
  • [41] Stable genetic transformation of Picea mariana (black spruce) via particle bombardment
    Charest, PJ
    Devantier, Y
    Lachance, D
    IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT, 1996, 32 (02) : 91 - 99
  • [42] Comparison of DNA marker and pedigree-based methods of genetic analysis of plantain and banana (Musa spp.) clones. I. estimation of genetic relationships
    A. Tenkouano
    J. H. Crouch
    H. K. Crouch
    D. Vuylsteke
    R. Ortiz
    Theoretical and Applied Genetics, 1999, 98 : 62 - 68
  • [43] Genetic transformation of Coffea canephora by particle bombardment
    Ribas, AF
    Kobayashi, AK
    Pereira, LFP
    Vieira, LGE
    BIOLOGIA PLANTARUM, 2005, 49 (04) : 493 - 497
  • [44] Genetic transformation of Cymbidium orchid by particle bombardment
    J. Yang
    H.-J. Lee
    D. H. Shin
    S. K. Oh
    J. H. Seon
    K. Y. Paek
    K.-H. Han
    Plant Cell Reports, 1999, 18 : 978 - 984
  • [45] Genetic transformation of Alstroemeria using particle bombardment
    Lin, HS
    van der Toorn, C
    Raemakers, KJJM
    Visser, RGF
    De Jeu, MJ
    Jacobsen, E
    MOLECULAR BREEDING, 2000, 6 (04) : 369 - 377
  • [46] Genetic transformation ofPinus taeda by particle bombardment
    Tang Wei
    Vanessa Samuels
    Journal of Forestry Research, 2002, 13 (2) : 91 - 97
  • [47] Genetic transformation of Alstroemeria using particle bombardment
    Hsueh-Shih Lin
    Caroline van der Toorn
    Krit J.J.M. Raemakers
    Richard G.F. Visser
    Marjo J. De Jeu
    Evert Jacobsen
    Molecular Breeding, 2000, 6 : 369 - 377
  • [48] Genetic transformation of Cymbidium orchid by particle bombardment
    Yang, J
    Lee, HJ
    Shin, DH
    Oh, SK
    Seon, JH
    Paek, KY
    Han, KH
    PLANT CELL REPORTS, 1999, 18 (12) : 978 - 984
  • [49] Comparison of DNA marker and pedigree-based methods of genetic analysis of plantain and banana (Musa spp.) clones.: I.: estimation of genetic relationships
    Tenkouano, A
    Crouch, JH
    Crouch, HK
    Vuylsteke, D
    Ortiz, R
    THEORETICAL AND APPLIED GENETICS, 1999, 98 (01) : 62 - 68
  • [50] Genetic diversity and population structure in banana (Musa spp.) breeding germplasm
    Akech, Violet
    Bengtsson, Therese
    Ortiz, Rodomiro
    Swennen, Rony
    Uwimana, Brigitte
    Ferreira, Claudia F.
    Amah, Delphine
    Amorim, Edson P.
    Blisset, Elizabeth
    van den Houwe, Ines
    Arinaitwe, Ivan K.
    Nice, Liana
    Bwesigye, Priver
    Tanksley, Steve
    Uma, Subbaraya
    Suthanthiram, Backiyarani
    Saraswathi, Marimuthu S.
    Mduma, Hassan
    Brown, Allan
    PLANT GENOME, 2024, 17 (03):
12345