GENETIC-TRANSFORMATION OF BANANA AND PLANTAIN (MUSA SPP) VIA PARTICLE BOMBARDMENT

被引：140

作者：

SAGI, L ^{[1
]}

PANIS, B ^{[1
]}

REMY, S ^{[1
]}

SCHOOFS, H ^{[1
]}

DESMET, K ^{[1
]}

SWENNEN, R ^{[1
]}

CAMMUE, BPA ^{[1
]}

机构：

[1] CATHOLIC UNIV LEUVEN,FA JANSSENS LAB GENET,B-3001 LOUVAIN,BELGIUM

来源：

BIO-TECHNOLOGY | 1995年 / 13卷 / 05期

关键词：

D O I：

10.1038/nbt0595-481

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We have developed a simple protocol to allow the production of transgenic banana plants. Foreign genes were delivered into embryogenic suspension cells using accelerated particles coated with DNA. Bombardment parameters were optimized for a modified particle gun resulting in high levels of transient expression of the beta-glucuronidase gene in both banana and plantain cells. Bombarded banana cells were selected with hygromycin and regenerated into plants. Molecular and histochemical characterization of transformants revealed the stable integration of the transferred genes into the banana genome.

引用

页码：481 / 485

页数：5

共 50 条

[31] Effect of temperature on symptom expression and reliability of banana streak badnavirus detection in naturally infected plantain and banana (Musa spp.)
Dahal, G
Hughes, JD
Thottappilly, G
Lockhart, BEL
PLANT DISEASE, 1998, 82 (01) : 16 - 21
[32] Genetic transformation of Linum by particle bombardment
Wijayanto, T
McHughen, A
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT, 1999, 35 (06) : 456 - 465
[33] Genetic transformation of Linum by particle bombardment
Wijayanto T.
McHughen A.
In Vitro Cellular & Developmental Biology - Plant, 1999, 35 (6) : 456 - 465
[34] Evaluation of micropropagated plantain and banana (Musa spp.) for banana streak badnavirus incidence under field and screenhouse conditions in Nigeria
Dahal, G
Gauhl, F
Pasberg-Gauhl, C
Hughes, JD
Thottappilly, G
Lockhart, BEL
ANNALS OF APPLIED BIOLOGY, 1999, 134 (02) : 181 - 191
[35] Factors Affecting the Adoption of Disease- Resistant Plantain and Banana ( Musa spp.) Hybrids in Nigeria
Aitchedji, C.
Tenkouano, A.
Coulibaly, O.
INTERNATIONAL CONFERENCE ON BANANA AND PLANTAIN IN AFRICA: HARNESSING INTERNATIONAL PARTNERSHIPS TO INCREASE RESEARCH IMPACT, 2010, 879 : 741 - 748
[36] STRATEGIES FOR VARIETY-INDEPENDENT GENETIC-TRANSFORMATION OF IMPORTANT CEREALS, LEGUMES AND WOODY SPECIES UTILIZING PARTICLE BOMBARDMENT
CHRISTOU, P
EUPHYTICA, 1995, 85 (1-3) : 13 - 27
[37] GENETIC-TRANSFORMATION OF WILLOWS (SALIX SPP) BY AGROBACTERIUM-TUMEFACIENS
VAHALA, T
STABEL, P
ERIKSSON, T
PLANT CELL REPORTS, 1989, 8 (02) : 55 - 58
[38] Robust CRISPR/Cas9 mediated genome editing tool for banana and plantain (Musa spp.)
Ntui, Valentine Otang
Tripathi, Jaindra Nath
Tripathi, Leena
CURRENT PLANT BIOLOGY, 2020, 21
[39] Benefits and Potential Use of Arbuscular Mycorrhizal Fungi (AMF) in Banana and Plantain (Musa spp.) Systems in Africa
Jefwa, J.
Vanlauwe, B.
Coyne, D.
van Asten, P.
Gaidashova, S.
Rurangwa, E.
Mwashasha, M.
Elsen, A.
INTERNATIONAL CONFERENCE ON BANANA AND PLANTAIN IN AFRICA: HARNESSING INTERNATIONAL PARTNERSHIPS TO INCREASE RESEARCH IMPACT, 2010, 879 : 479 - 486
[40] Research-Extension-Farmers Linkage System on Banana and Plantain (Musa spp.) in Nigeria: the Diffusion of Innovations
Oladele, O. I.
INTERNATIONAL CONFERENCE ON BANANA AND PLANTAIN IN AFRICA: HARNESSING INTERNATIONAL PARTNERSHIPS TO INCREASE RESEARCH IMPACT, 2010, 879 : 837 - 842

← 1 2 3 4 5 →