GENETIC-TRANSFORMATION OF BANANA AND PLANTAIN (MUSA SPP) VIA PARTICLE BOMBARDMENT

被引：140
|
作者：
SAGI, L [1 ]
PANIS, B [1 ]
REMY, S [1 ]
SCHOOFS, H [1 ]
DESMET, K [1 ]
SWENNEN, R [1 ]
CAMMUE, BPA [1 ]
机构：
[1] CATHOLIC UNIV LEUVEN,FA JANSSENS LAB GENET,B-3001 LOUVAIN,BELGIUM
来源：
BIO-TECHNOLOGY | 1995年 / 13卷 / 05期
关键词：
D O I：
10.1038/nbt0595-481
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
We have developed a simple protocol to allow the production of transgenic banana plants. Foreign genes were delivered into embryogenic suspension cells using accelerated particles coated with DNA. Bombardment parameters were optimized for a modified particle gun resulting in high levels of transient expression of the beta-glucuronidase gene in both banana and plantain cells. Bombarded banana cells were selected with hygromycin and regenerated into plants. Molecular and histochemical characterization of transformants revealed the stable integration of the transferred genes into the banana genome.
引用
收藏
页码：481 / 485
页数：5
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