Data of a fluorescent imaging-based analysis of anti-cancer drug effects on three-dimensional cultures of breast cancer cells

被引：2

作者：

Itou, Junji ^{[1
]}

Tanaka, Sunao ^{[1
]}

Li, Wenzhao ^{[1
]}

Matsumoto, Yoshiaki ^{[1
]}

Sato, Fumiaki ^{[1
]}

Toi, Masakazu ^{[1
]}

机构：

[1] Kyoto Univ, Grad Sch Med, Dept Breast Surg, Sakyo Ku, 54 Shogoin Kawahara Cho, Kyoto 6068507, Japan

来源：

DATA IN BRIEF | 2015年 / 5卷

关键词：

Anti-cancer drug; Breast cancer; Live imaging; Photoconvertible fluorescent protein; Pulse labeling; Three-dimensional culture;

D O I：

10.1016/j.dib.2015.09.037

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Three-dimensional (3D) cell culture is a powerful tool to study cell growth under 3D condition. To perform a simple test for anticancer drugs in 3D culture, visualization of non-proliferated cells is required. We propose a fluorescent imaging-based assay to analyze cancer cell proliferation in 3D culture. We used a pulse-labeling technique with a photoconvertible fluorescent protein Kaede to identify non-proliferated cells. This assay allows us to observe change in cell proliferation in 3D culture by simple imaging. Using this assay, we obtained the data of the effects of anti-cancer drugs, 5-fluorouracil and PD0332991 in a breast cancer cell line, MCF-7. (C) 2015 The Authors. Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/40).

引用

页码：429 / 433

页数：5

共 5 条

[1] PD 0332991, a selective cyclin D kinase 4/6 inhibitor, preferentially inhibits proliferation of luminal estrogen receptor-positive human breast cancer cell lines in vitro [J].