SEPARATION OF GLYCOPROTEIN-N-GLYCANS BY HIGH-PH ANION-EXCHANGE CHROMATOGRAPHY

被引：33

作者：

PFEIFFER, G

GEYER, H

GEYER, R

KALSNER, I

WENDORF, P

机构：

[1] UNIV GIESSEN KLINIKUM,INST BIOCHEM,FRIEDRICHSTR 24,W-6300 GIESSEN,GERMANY

[2] ERNST BOEHRINGER INST ARZNEIMITTELFORSCH,A-1121 VIENNA,AUSTRIA

[3] UNIV GIESSEN KLINIKUM,INST MED MIKROBIOL,W-6300 GIESSEN,GERMANY

来源：

BIOMEDICAL CHROMATOGRAPHY | 1990年 / 4卷 / 05期

关键词：

D O I：

10.1002/bmc.1130040505

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A series of high‐mannose and complex type glycoprotein‐N‐glycans was subjected to high‐pH anion‐exchange chromatography. The results revealed that this method represents a useful tool for analytical characterization of single oligosaccharides as well as preparative separation of complex mixtures of carbohydrate side‐chains. On the other hand, it became evident that in several cases a combination of different chromatographic techniques is required for efficient separation of individual oligosaccharide species. Copyright © 1990 John Wiley & Sons, Ltd.

引用

页码：193 / 199

页数：7

共 29 条

[21]

STRUBE KH, 1988, J BIOL CHEM, V263, P3762

[22] CARBOHYDRATE STRUCTURE OF GLYCOPROTEIN-65 ENCODED BY THE POLYCYTHEMIA-INDUCING STRAIN OF FRIEND SPLEEN FOCUS-FORMING VIRUS [J].