SEPARATION OF GLYCOPROTEIN-N-GLYCANS BY HIGH-PH ANION-EXCHANGE CHROMATOGRAPHY

被引：33
作者：
PFEIFFER, G
GEYER, H
GEYER, R
KALSNER, I
WENDORF, P
机构：
[1] UNIV GIESSEN KLINIKUM,INST BIOCHEM,FRIEDRICHSTR 24,W-6300 GIESSEN,GERMANY
[2] ERNST BOEHRINGER INST ARZNEIMITTELFORSCH,A-1121 VIENNA,AUSTRIA
[3] UNIV GIESSEN KLINIKUM,INST MED MIKROBIOL,W-6300 GIESSEN,GERMANY
来源：
BIOMEDICAL CHROMATOGRAPHY | 1990年 / 4卷 / 05期
关键词：
D O I：
10.1002/bmc.1130040505
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A series of high‐mannose and complex type glycoprotein‐N‐glycans was subjected to high‐pH anion‐exchange chromatography. The results revealed that this method represents a useful tool for analytical characterization of single oligosaccharides as well as preparative separation of complex mixtures of carbohydrate side‐chains. On the other hand, it became evident that in several cases a combination of different chromatographic techniques is required for efficient separation of individual oligosaccharide species. Copyright © 1990 John Wiley & Sons, Ltd.
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页码：193 / 199
页数：7
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