SURFACE MARKERS ON HUMAN B-LYMPHOCYTES AND T-LYMPHOCYTES .9. 2-COLOR IMMUNOFLUORESCENCE STUDIES ON ASSOCIATION BETWEEN EBV RECEPTORS AND COMPLEMENT RECEPTORS ON SURFACE OF LYMPHOID-CELL LINES

Receptors for the 3rd component of complement (C3) were demonstrated on the surface of established human lymphoid [Burkitt lymphoma] cell lines by membrane fluorescence tests with FITC [fluorescein isothiocyanate] or TRIC [tetramethylrodamine isothiocyanate]-conjugated antibodies against human C3. Two color fluorescence staining of EBV [Epstein-Barr virus] receptors and C3 receptors showed complete overlapping of green and red fluorescence. Capping of the EBV receptor induced cocapping of the C3 receptor and vice versa. There was neither overlapping nor cocapping when EBV or C3 receptors were examined in relation to Fc receptors, surface IgM [immunoglobulin M] or .beta.2-microglobulin. The kinetic pattern of EBV receptor capping was identical to the pattern of C3 receptor capping, but differed from the pattern of IgM capping. These results suggest a close association between EBV and C3 receptors on the human B [bone marrow-derived] lymphocyte.