CONTINUOUS-FLOW MONITOR SYSTEM FOR DETECTION OF UV ABSORBANCE 14C AND 3H IN EFFLUENT OF A COLUMN CHROMATOGRAM

被引：32

作者：

HUNT, JA

机构：

[1] Department of Genetics, School of Medicine, University of Hawaii, Honolulu

来源：

ANALYTICAL BIOCHEMISTRY | 1968年 / 23卷 / 02期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/0003-2697(68)90360-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A flow monitor system is described that permits a column chromatogram to be monitored at two or more wavelengths spectrophotometrically and for radioactivity of two different isotopes. Radioactivity is measured on part of the effluent, which is mixed with a dioxane-based scintillator for passage through a polyethylene coil flow cell in a dual-channel liquid scintillation flow monitor. An efficiency of up to 14% is obtainable for 3H and 70% for 14C. © 1968.

引用

页码：289 / &

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