SUBTYPING OF HEPATITIS-C VIRUS ISOLATES BY A LINE PROBE ASSAY USING HYBRIDIZATION

被引：29

作者：

ANDONOV, A ^{[1
]}

CHAUDHARY, RK ^{[1
]}

机构：

[1] HLTH CANADA,LAB CTR DIS CONTROL,BUR MICROBIOL,VIRAL HEPATITIS LAB,OTTAWA,ON,CANADA

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 1995年 / 33卷 / 01期

关键词：

D O I：

10.1128/JCM.33.1.254-256.1995

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A reverse hybridization test (Inno-LiPA HCV; Innogenetics, N.V., Zwijnaarde, Belgium) was used for typing hepatitis C virus. All 38 samples, typed by PCR with primers from core and NS5 genes, were also genotyped by this test. Of the samples, 33 (87%) had the same subtypes by both assays. The correlations between PCR and Inno-LiPA for individual types were 77% for type I (1a), 90% for type II (1b), 100% for type III (2a), 100% for type IV (2b), and 100% for type V (3a). One of the type III (2a) samples also reacted with type I (1a) probes in the Inno-LiPA test.

引用

页码：254 / 256

页数：3

共 14 条

[1] GENOTYPING OF CANADIAN HEPATITIS-C VIRUS ISOLATES BY PCR [J].

ANDONOV, A ;

CHAUDHARY, RK .

JOURNAL OF CLINICAL MICROBIOLOGY, 1994, 32 (08) :2031-2034

[2] AT LEAST 12 GENOTYPES OF HEPATITIS-C VIRUS PREDICTED BY SEQUENCE-ANALYSIS OF THE PUTATIVE E1-GENE OF ISOLATES COLLECTED WORLDWIDE [J].

BUKH, J ;

PURCELL, RH ;

MILLER, RH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (17) :8234-8238

[3] AT LEAST 5 RELATED, BUT DISTINCT, HEPATITIS-C VIRAL GENOTYPES EXIST [J].

CHA, TA ;

BEALL, E ;

IRVINE, B ;

KOLBERG, J ;

CHIEN, D ;

KUO, G ;

URDEA, MS .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (15) :7144-7148

[4] GENOTYPIC SUBTYPING OF HEPATITIS-C VIRUS [J].

CHAYAMA, K ;

TSUBOTA, A ;

ARASE, Y ;

SAITOH, S ;

KOIDA, I ;

IKEDA, K ;

MATSUMOTO, T ;

KOBAYASHI, M ;

IWASAKI, S ;

KOYAMA, S ;

MORINAGA, T ;

KUMADA, H .

JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY, 1993, 8 (02) :150-156

[5] SINGLE-STEP METHOD OF RNA ISOLATION BY ACID GUANIDINIUM THIOCYANATE PHENOL CHLOROFORM EXTRACTION [J].

CHOMCZYNSKI, P ;

SACCHI, N .

ANALYTICAL BIOCHEMISTRY, 1987, 162 (01) :156-159

[6]

KAANAI K, 1992, LANCET, V339, P1543

[7]

MCOMISH F, 1992, TRANSFUSION, V33, P7

[8] TYPING OF HEPATITIS-C VIRUS GENOMES BY RESTRICTION-FRAGMENT-LENGTH-POLYMORPHISM [J].

NAKAO, T ;

ENOMOTO, N ;

TAKADA, N ;

TAKADA, A ;

DATE, T .

JOURNAL OF GENERAL VIROLOGY, 1991, 72 :2105-2112

[9] TYPING HEPATITIS-C VIRUS BY POLYMERASE CHAIN-REACTION WITH TYPE-SPECIFIC PRIMERS - APPLICATION TO CLINICAL SURVEYS AND TRACING INFECTIOUS SOURCES [J].

OKAMOTO, H ;

SUGIYAMA, Y ;

OKADA, S ;

KURAI, K ;

AKAHANE, Y ;

SUGAI, Y ;

TANAKA, T ;

SATO, K ;

TSUDA, F ;

MIYAKAWA, Y ;

MAYUMI, M .

JOURNAL OF GENERAL VIROLOGY, 1992, 73 :673-679

[10] CHARACTERIZATION OF THE GENOMIC SEQUENCE OF TYPE-V (OR 3A) HEPATITIS-C VIRUS ISOLATES AND PCR PRIMERS FOR SPECIFIC DETECTION [J].

OKAMOTO, H ;

TOKITA, H ;

SAKAMOTO, M ;

HORIKITA, M ;

KOJIMA, M ;

IIZUKA, H ;

MISHIRO, S .

JOURNAL OF GENERAL VIROLOGY, 1993, 74 :2385-2390

← 1 2 →