DNA-BINDING ACTIVITY OF NTRC FROM RHIZOBIUM GROWN ON DIFFERENT NITROGEN-SOURCES

被引：15

作者：

PATRIARCA, EJ ^{[1
]}

RICCIO, A ^{[1
]}

COLONNAROMANO, S ^{[1
]}

DEFEZ, R ^{[1
]}

IACCARINO, M ^{[1
]}

机构：

[1] CNR, INT INST GENET & BIOPHYS, I-80125 NAPLES, ITALY

来源：

FEBS LETTERS | 1994年 / 354卷 / 01期

关键词：

2-COMPONENT SYSTEM; NITROGEN REGULATION; GEL RETARDATION;

D O I：

10.1016/0014-5793(94)01094-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The DNA-binding activity of the NtrC protein can be demonstrated in gel retardation assays with concentrated protein extracts of Rhizobium etli. Using extracts from either the wild type or a ntrC mutant strain and an antiserum raised against the NtrC protein, we demonstrate specific binding of NtrC to the upstream regulatory region of the glnII gene, where two putative NtrC-binding sites are present. KNO3-grown bacteria contain less NtrC protein and more NtrC-binding activity than NH4Cl-grown bacteria, thus showing that with this protocol it is possible to detect changes in NtrC-binding activity. The advantages of this assay system in comparison with that using pure proteins is discussed.

引用

页码：89 / 92

页数：4

共 25 条

[21] PROBING THE ESCHERICHIA-COLI GLNALG UPSTREAM ACTIVATION MECHANISM INVIVO [J].

SASSEDWIGHT, S ;

GRALLA, JD .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (23) :8934-8938

[22] RECLASSIFICATION OF AMERICAN RHIZOBIUM-LEGUMINOSARUM BIOVAR PHASEOLI TYPE-I STRAINS AS RHIZOBIUM-ETLI SP-NOV [J].

SEGOVIA, L ;

YOUNG, JPW ;

MARTINEZROMERO, E .

INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1993, 43 (02) :374-377

[23] PROTEIN-PHOSPHORYLATION AND REGULATION OF ADAPTIVE RESPONSES IN BACTERIA [J].

STOCK, JB ;

NINFA, AJ ;

STOCK, AM .

MICROBIOLOGICAL REVIEWS, 1989, 53 (04) :450-490

[24]

THONY B, 1989, FEMS MICROBIOL LETT, V63, P341

[25] PHOSPHORYLATION OF NITROGEN REGULATOR-I OF ESCHERICHIA-COLI INDUCES STRONG COOPERATIVE BINDING TO DNA ESSENTIAL FOR ACTIVATION OF TRANSCRIPTION [J].

WEISS, V ;

CLAVERIEMARTIN, F ;

MAGASANIK, B .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (11) :5088-5092

← 1 2 3 →