A DISORDER OF COLLAGEN BIOSYNTHESIS IN PATIENTS WITH CEREBRAL-ARTERY ANEURYSM

被引:15
作者
MAJAMAA, K [1 ]
MYLLYLA, VV [1 ]
机构
[1] UNIV OULU,DEPT MED BIOCHEM,OULU,FINLAND
基金
芬兰科学院; 英国医学研究理事会;
关键词
COLLAGEN; COLLAGEN BIOSYNTHESIS; COLLAGEN SECRETION; CAROTID ARTERY; CEREBRAL ANEURYSM; (HUMAN);
D O I
10.1016/0925-4439(93)90120-P
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
12 patients with subarachnoid hemorrhage due to rupture of a cerebral aneurysm were examined clinically for symptoms and signs of a connective tissue disorder and biochemically for details of the biosynthesis of collagen. No uniform clinical pattern of any connective tissue disorder was seen in these patients, although selected signs were observed. Skin fibroblast cultures were then established. The rate of procollagen production in two cell lines was reduced by 40% and 50%, respectively, and the intracellular accumulation of hydroxy[C-14]proline (as a percentage of total hydroxy[C-14]proline) was increased by 70% in each relative to eight control cell lines. No difference was found in the degree of intracellular degradation of procollagen. After pulse-labelling, however, the radioactive procollagen was secreted into the medium in 1 h in the control cells, but required at feast 3 h in the two aneurysm patient cell lines. The results, thus, suggest that delayed secretion of procollagen rather than increased intracellular degradation led to the reduction in the rate of procollagen synthesis in these two fibroblast lines from patients with cerebral artery aneurysm.
引用
收藏
页码:48 / 52
页数:5
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