PREFERENTIAL NUCLEOSOME ASSEMBLY AT DNA TRIPLET REPEATS FROM THE MYOTONIC-DYSTROPHY GENE

被引：198

作者：

WANG, YH

AMIRHAERI, S

KANG, S

WELLS, RD

GRIFFITH, JD

机构：

[1] UNIV N CAROLINA,LINEBERGER CANC RES CTR,CHAPEL HILL,NC 27599

[2] TEXAS A&M UNIV,INST BIOSCI & TECHNOL,HOUSTON,TX 77030

来源：

SCIENCE | 1994年 / 265卷 / 5172期

关键词：

D O I：

10.1126/science.8036515

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The expansion of CTG repeats in DNA occurs in or near genes involved in several human diseases, including myotonic dystrophy and Huntington's disease. Nucleosomes, the basic structural element of chromosomes, consist of 146 base pairs of DNA coiled about an octamer of histone proteins and mediate general transcriptional repression. Electron microscopy was used to examine in vitro the nucleosome assembly of DNA containing repeating CTG triplets. The efficiency of nucleosome formation increased with expanded triplet blocks, suggesting that such blocks may repress transcription through the creation of stable nucleosomes.

引用

收藏

页码：669 / 671

页数：3

相关论文

共 25 条

[1]

AMIRHAERI S, UNPUB

[2] ABSENCE OF MYOTONIC-DYSTROPHY PROTEIN-KINASE (DMPK) MESSENGER-RNA AS A RESULT OF A TRIPLET REPEAT EXPANSION IN MYOTONIC-DYSTROPHY [J].

CARANGO, P ;

NOBLE, JE ;

MARKS, HG ;

FUNANAGE, VL .

GENOMICS, 1993, 18 (02) :340-348

[3] TRIPLET REPEAT MUTATIONS IN HUMAN-DISEASE [J].

CASKEY, CT ;

PIZZUTI, A ;

FU, YH ;

FENWICK, RG ;

NELSON, DL .

SCIENCE, 1992, 256 (5058) :784-789

[4] AN UNSTABLE TRIPLET REPEAT IN A GENE RELATED TO MYOTONIC MUSCULAR-DYSTROPHY [J].

FU, YH ;

PIZZUTI, A ;

FENWICK, RG ;

KING, J ;

RAJNARAYAN, S ;

DUNNE, PW ;

DUBEL, J ;

NASSER, GA ;

ASHIZAWA, T ;

DEJONG, P ;

WIERINGA, B ;

KORNELUK, R ;

PERRYMAN, MB ;

EPSTEIN, HF ;

CASKEY, CT .

SCIENCE, 1992, 255 (5049) :1256-1258

[5] VARIATION OF THE CGG REPEAT AT THE FRAGILE-X SITE RESULTS IN GENETIC INSTABILITY - RESOLUTION OF THE SHERMAN PARADOX [J].

FU, YH ;

KUHL, DPA ;

PIZZUTI, A ;

PIERETTI, M ;

SUTCLIFFE, JS ;

RICHARDS, S ;

VERKERK, AJMH ;

HOLDEN, JJA ;

FENWICK, RG ;

WARREN, ST ;

OOSTRA, BA ;

NELSON, DL ;

CASKEY, CT .

CELL, 1991, 67 (06) :1047-1058

[6] ELECTRON-MICROSCOPE VISUALIZATION OF CHROMATIN AND OTHER DNA-PROTEIN COMPLEXES [J].

GRIFFITH, JD ;

CHRISTIANSEN, G .

ANNUAL REVIEW OF BIOPHYSICS AND BIOENGINEERING, 1978, 7 :19-35

[7] THE HISTONE CORE EXERTS A DOMINANT CONSTRAINT ON THE STRUCTURE OF DNA IN A NUCLEOSOME [J].

HAYES, JJ ;

BASHKIN, J ;

TULLIUS, TD ;

WOLFFE, AP .

BIOCHEMISTRY, 1991, 30 (34) :8434-8440

[8] THE STRUCTURE OF DNA IN A NUCLEOSOME [J].

HAYES, JJ ;

TULLIUS, TD ;

WOLFFE, AP .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (19) :7405-7409

[9] HISTONE CONTRIBUTIONS TO THE STRUCTURE OF DNA IN THE NUCLEOSOME [J].

HAYES, JJ ;

CLARK, DJ ;

WOLFFE, AP .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1991, 88 (15) :6829-6833

[10] THE TERMINUS OF SV40 DNA-REPLICATION AND TRANSCRIPTION CONTAINS A SHARP SEQUENCE-DIRECTED CURVE [J].

HSIEH, CH ;

GRIFFITH, JD .

CELL, 1988, 52 (04) :535-544