SELECTIVE INTERACTION OF FERRICYANIDE WITH CLUSTER-I OF CLOSTRIDIUM-PASTEURIANUM 2[FE4S4] FERREDOXIN

被引：19
|
作者：
BERTINI, I [1 ]
BRIGANTI, F [1 ]
CALZOLAI, L [1 ]
MESSORI, L [1 ]
SCOZZAFAVA, A [1 ]
机构：
[1] UNIV FLORENCE, DEPT CHEM, VIA GINO CAPPONI 7, I-50121 FLORENCE, ITALY
来源：
FEBS LETTERS | 1993年 / 332卷 / 03期
关键词：
FERREDOXIN; NMR; CLOSTRIDIUM-PASTEURIANUM;
D O I：
10.1016/0014-5793(93)80647-D
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Treatment of Clostridium pasteurianum ferredoxin (CpFd) with stoichiometric amounts of potassium ferricyanide results in the specific conversion of cluster I into a Fe3S4+ species while leaving cluster II unaltered. Ferricyanide-treated CpFd derivative has been purified and characterized through biochemical and spectroscopical techniques. The cluster conversion process is reversible and reconstitution of native CpFd has been afforded under appropriate conditions.
引用
收藏
页码：268 / 272
页数：5
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