ORIGIN OF SINGLE PRONUCLEATED HUMAN ZYGOTES

被引：31
作者：
MUNNE, S
TANG, YX
GRIFO, J
COHEN, J
机构：
[1] The Gamete and Embryo Research Laboratory, Center for Reproductive Medicine and Infertility, Department of Obstetrics and Gynecology, New York Hospital-Cornell University Medical Center, New York, 10021
来源：
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS | 1993年 / 10卷 / 04期
关键词：
IN-VITRO FERTILIZATION; POLYMERASE CHAIN REACTION; IN-SITU HYBRIDIZATION; SINGLE PRONUCLEATED ZYGOTES; PREIMPLANTATION GENETICS;
D O I：
10.1007/BF01204942
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
Purpose: Diploidy in embryos developing from single pronucleated zygotes can occur following parthenogenetic activation or by asynchronous inflation of pronuclei following fertilization. To distinguish between these two mechanisms, sexing was performed. Results: The presence of a Y chromosome in the embryo was considered proof that fertilization had occurred. Twenty-one dividing embryos originating from single pronucleated zygotes were analyzed using the polymerase chain reaction or fluorescence in situ hybridization. In total, 43% (9121) of the embryos showed Y chromosomes. Conclusion: It can be extrapolated that more than 80% of them originated from fertilized eggs.
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收藏
页码：276 / 279
页数：4
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