SPECTROPHOTOMETRIC DETERMINATION OF LYSINE IN THE PRESENCE OF OTHER AMINO-ACIDS AND PROTEINS USING FURFURALDEHYDE

被引：3
|
作者：
MOLNARPERL, I
PINTERSZAKACS, M
机构：
[1] Institute of Inorganic and Analytical Chemistry, L. Eötvös University, H-1518 Budapest
来源：
ANALYTICA CHIMICA ACTA | 1992年 / 257卷 / 02期
关键词：
SPECTROPHOTOMETRY; AMINO ACIDS; LYSINE; PROTEINS;
D O I：
10.1016/0003-2670(92)85173-4
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
The selective reaction of lysine, delta-hydroxylysine, ornithine and alpha,epsilon-diaminopimelic acid with furfuraldehyde, in anhydrous media, was investigated at 80, 85, 90 and 95-degrees-C by spectrophotometry, in the presence of various additional materials, such as potassium pyrosulphite, ascorbic acid and lead(II) acetate. Second-order rate constants and molar absorptivities were determined. Optimum conditions were established for the selective determination of lysine in the presence of intact proteins or in protein hydrolysates in the presence of a wide range of other amino acids including ornithine. Under optimum conditions, in the concentration range 2 x 10(-8)-3 x 10(-7) M, molar absorptivities and reproducibility data were measured with a standard error of 0.9-5.0%, depending on the procedure applied and on the amount of lysine present.
引用
收藏
页码：209 / 216
页数：8
相关论文
共 50 条
  • [21] COLORIMETRIC DETERMINATION OF SOME AMINO-ACIDS CONTAINING A SULFUR GROUP
    ELBRASHY, AM
    ALGHANNAM, SM
    PHARMACY WORLD & SCIENCE, 1995, 17 (02): : 54 - 57
  • [22] THE SOLID-STATE CATALYTIC SYNTHESIS OF TRITIUM-LABELED AMINO-ACIDS, PEPTIDES AND PROTEINS
    ZOLOTAREV, YA
    DOROKHOVA, EM
    NEZAVIBATKO, VN
    BORISOV, YA
    ROSENBERG, SG
    VELIKODVORSKAIA, GA
    NEUMIVAKIN, LV
    ZVERLOV, VV
    MYASOEDOV, NF
    AMINO ACIDS, 1995, 8 (04) : 353 - 365
  • [23] DIRECT DETERMINATION OF AMINO-ACIDS BY CAPILLARY ELECTROPHORESIS ELECTROCHEMISTRY USING A COPPER MICROELECTRODE AND ZWITTERIONIC BUFFERS
    ZHOU, J
    LUNTE, SM
    ELECTROPHORESIS, 1995, 16 (04) : 498 - 503
  • [24] EFFECTS OF ALKALI TREATMENT ON THE INVITRO DIGESTIBILITY OF PROTEINS AND THE RELEASE OF AMINO-ACIDS
    SAVOIE, L
    PARENT, G
    GALIBOIS, I
    JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, 1991, 56 (03) : 363 - 372
  • [25] THE CONTRIBUTION FROM THE CHOROID-PLEXUS AND THE PERIVENTRICULAR CNS TO AMINO-ACIDS AND PROTEINS IN THE HUMAN CSF
    HAMBERGER, A
    NYSTROM, B
    SILVENIUS, H
    WIKKELSO, C
    NEUROCHEMICAL RESEARCH, 1990, 15 (03) : 307 - 312
  • [26] DETERMINATION OF SOLUBLE LIGNIN AND PROTEINS IN THE PRESENCE OF EACH OTHER
    CHAUVET, JM
    LAMY, F
    ANALYTICA CHIMICA ACTA, 1990, 235 (02) : 299 - 306
  • [27] INDIRECT DETERMINATION OF AMINO-ACIDS BY MOLECULAR-EMISSION CAVITY ANALYSIS
    ALZAMIL, Z
    SULTAN, SM
    HASSAN, YA
    ANALYTICA CHIMICA ACTA, 1990, 239 (01) : 161 - 164
  • [28] Application of Raman spectroscopy for the determination of proteins denaturation and amino acids decomposition temperature
    Ojeda-Galvan, H. J.
    Hernandez-Arteaga, A. C.
    Rodriguez-Aranda, M. C.
    Toro-Vazquez, J. F.
    Cruz-Gonzalez, N.
    Ortiz-Chavez, S.
    Comas-Garcia, M.
    Rodriguez, A. G.
    Navarro-Contreras, H. R.
    SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, 2023, 285
  • [29] ENDOGENOUS FLOW OF TOTAL LYSINE AND OTHER AMINO-ACIDS AT THE DISTAL ILEUM OF THE PROTEIN-FED OR PEPTIDE-FED RAT - THE CHEMICAL LABELING OF GELATIN PROTEIN BY TRANSFORMATION OF LYSINE TO HOMOARGININE
    MOUGHAN, PJ
    RUTHERFURD, SM
    JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE, 1990, 52 (02) : 179 - 192
  • [30] CHEMILUMINESCENCE DETECTION OF AMINO-ACIDS, PEPTIDES, AND PROTEINS USING TRIS-2,2'-BIPYRIDINE RUTHENIUM(III)
    HE, L
    COX, KA
    DANIELSON, ND
    ANALYTICAL LETTERS, 1990, 23 (02) : 195 - 210
12345