V(D)J RECOMBINATION IN MOUSE THYMOCYTES - DOUBLE-STRAND BREAKS NEAR T-CELL RECEPTOR DELTA REARRANGEMENT SIGNALS

被引：168

作者：

ROTH, DB ^{[1
]}

NAKAJIMA, PB ^{[1
]}

MENETSKI, JP ^{[1
]}

BOSMA, MJ ^{[1
]}

GELLERT, M ^{[1
]}

机构：

[1] FOX CHASE CANC INST, INST CANC RES, PHILADELPHIA, PA 19111 USA

来源：

CELL | 1992年 / 69卷 / 01期

关键词：

D O I：

10.1016/0092-8674(92)90117-U

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In the murine T cell receptor delta-locus, V(D)J recombination events frequently involve the D2 and J1 elements. Here we report the presence of double-strand breaks at recombination signals flanking D2 in approximately 2% of thymus DNA. An excised linear species containing the sequences between D2 and J1 and a circular product of the joining of D2 and J1 recombination signals were also found. Although broken molecules with signal ends were detected, no species with coding ends could be identified. Observation of these broken molecules in thymus, but not in liver or spleen, provides the first direct evidence for an association between specific cleavage of chromosomal DNA and recombination in mammalian cells, and supports a breakage-reunion model of V(D)J recombination.

引用

页码：41 / 53

页数：13

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