A SIMPLE AND RAPID EXPERIMENTAL PROTOCOL FOR STUDIES OF NUCLEIC-ACIDS METABOLISM AND THEIR BASE COMPOSITION

被引:1
|
作者
LIRAS, A [1 ]
机构
[1] UNIV AUTONOMA MADRID,FAC MED,MADRID 34,SPAIN
来源
JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 1991年 / 23卷 / 02期
关键词
HPLC; NUCLEIC ACID METABOLISM; BASE COMPOSITION; ARTEMIA;
D O I
10.1016/0165-022X(91)90066-6
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A suitable, simple and rapid protocol for metabolic studies of nucleic acids and determining their base composition, using reversed-phase high-performance liquid chromatography is described. Modified classic methods of isolation of the nucleic acids fraction from a biological material, in our particular case Artemia sp., were used. Then analysis of their constituents and the incorporated radioactivity, after hydrolytic processes, was performed by high-performance liquid chromatography under isocratic conditions, with 9 min total retention time. This method may be applied in several aspects of nucleic acids research, such as molecular cloning or metabolic and phylogenetic studies.
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页码:189 / 192
页数:4
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