A SIMPLE AND RAPID EXPERIMENTAL PROTOCOL FOR STUDIES OF NUCLEIC-ACIDS METABOLISM AND THEIR BASE COMPOSITION

被引：1

作者：

LIRAS, A ^{[1
]}

机构：

[1] UNIV AUTONOMA MADRID,FAC MED,MADRID 34,SPAIN

来源：

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 1991年 / 23卷 / 02期

关键词：

HPLC; NUCLEIC ACID METABOLISM; BASE COMPOSITION; ARTEMIA;

D O I：

10.1016/0165-022X(91)90066-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A suitable, simple and rapid protocol for metabolic studies of nucleic acids and determining their base composition, using reversed-phase high-performance liquid chromatography is described. Modified classic methods of isolation of the nucleic acids fraction from a biological material, in our particular case Artemia sp., were used. Then analysis of their constituents and the incorporated radioactivity, after hydrolytic processes, was performed by high-performance liquid chromatography under isocratic conditions, with 9 min total retention time. This method may be applied in several aspects of nucleic acids research, such as molecular cloning or metabolic and phylogenetic studies.

引用

页码：189 / 192

页数：4