POLYMERIC COATINGS IN PACKED FUSED-SILICA MICRO-LC COLUMNS

Different polymer-coated stationary phases have been evaluated in micro-LC: Nucleosil-100-5-C1-PMSC18 (C18-substituted polymethylsiloxane, C1-deactivated with trimethylsilylenolate) and Nucleosil-100-5-C1-PMSCN (cyanopropyl-substituted polymethylsiloxane, C1-deactivated) in the reversed phase mode and Nucleosil-100-5-PEG (polyethylene glycol-coated) in the normal phase mode. Fused silica tubing of 320-mu-m i.d. was slurry packed with suspensions optimized for each packing material. The micropacked columns were studied for efficiency and selectivity. The silanol activities of the polymer coated phases were compared to monomeric phases for the reversed phase mode and to silica for the normal phase mode.