AN ELECTROCHEMICALLY GROWN POLYMER AS AN IMMOBILIZATION MATRIX FOR WHOLE CELLS - APPLICATION IN AN AMPEROMETRIC DOPAMINE SENSOR

被引：58

作者：

DESHPANDE, MV ^{[1
]}

HALL, EAH ^{[1
]}

机构：

[1] UNIV CAMBRIDGE,INST BIOTECHNOL,DOWNING ST,CAMBRIDGE CB2 3EF,ENGLAND

来源：

BIOSENSORS & BIOELECTRONICS | 1990年 / 5卷 / 06期

关键词：

catecholamines; dopamine; immobilisation; polypyrrole; sensor; whole cell;

D O I：

10.1016/0956-5663(90)80033-A

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

A method is described for the entrapment of whole banana cells in a polypyrrole film electrochemically deposited on a gold electrode. The polyphenol oxidase activity of the incorporated whole cells in the polymer was studied using dopamine as the major substrate. This amperometric-based probe was compared for the measurement of dopamine and related catecholamines. It exhibited high biocatalytic activity, good response time, favourable selectivity and reusability. © 1990.

引用

页码：431 / 448

页数：18

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