Objective: this study describes the fauna and the behavior of Anopheles species in order to understand the link in the entomological malaria transmission in the Municipality of Colniza, State of Mato Grosso. Methods: data were collected from the Information System of Epidemiological Surveillance of Malaria (Sivep-malaria), and from entomological reports of the city in March, May, July and September 2008. Results: presence of Anopheles darlingi, An. benarrochi, An. mediopunctatus, An. triannulatus and An. nuneztovari was observed; An. darlingi (97.5%) was the predominant species, captured in all months covered by the study but with high density in May, with increased activity in the evening and at home. Conclusion: in five present species collected, the main vector species of malaria, An. darlingi, was predominant in peridomiciliar area, with high density in May; such information allow to direct actions of control considering aspects related to the behavior of the vectors.