ESTIMATION OF ENZYMICALLY PRODUCED ORTHOPHOSPHATE IN PRESENCE OF CYSTEINE AND ADENOSINE TRIPHOSPHATE

被引：267

作者：

RATHBUN, WB

BETLACH, MV

机构：

[1] Department of Ophthalmology, University of Minnesota, Minneapolis

来源：

ANALYTICAL BIOCHEMISTRY | 1969年 / 28卷 / 1-3期

关键词：

D O I：

10.1016/0003-2697(69)90198-5

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method utilizing phosphomolybdate is described for determination of enzymically formed orthophosphate in the presence of high concentrations of cysteine, ATP, and ADP. This sensitive procedure allows the rapid estimation of phosphate for the large number of samples that are usually encountered in enzyme studies. The variables of the procedure were examined in detail, and the method which evolved minimizes errors in practical enzyme assay procedures. © 1969.

引用

页码：436 / &

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