AN ELECTROPHORETIC ANALYSIS OF PROTEIN COMPONENTS OF HUMAN PAROTID SALIVA

被引：50
作者：
STEINER, JC
KELLER, PJ
机构：
[1] Department of Oral Biology, School of Dentistry, University of Washington, Seattle
来源：
ARCHIVES OF ORAL BIOLOGY | 1968年 / 13卷 / 10期
关键词：
D O I：
10.1016/0003-9969(68)90077-0
中图分类号：
R78 [口腔科学];
学科分类号：
1003 ;
摘要：
Fresh samples of parotid saliva from one subject were analyzed by complementary anionic and cationic polyacrylamide disc gel electrophoresis systems. Nineteen protein components were evidenced with Amido Black, whereas a total of 30 discrete components were revealed by the more sensitive dye, Coomassie Brilliant Blue. The polyacrylamide gel patterns were qualitatively reproducible and have been described by grouping bands into regions designated A or A′, B or B′, C and D. Regions A and A′ contain protein and neutral carbohydrate. Regions B and B′ contain all of the α-amylase activity of parotid saliva, in the form of four or more isoenzymes. The dominant B-band also possesses acidic carbohydrate material. Region C contains five acidic components one of which contains acidic carbohydrate. Five cationic components are consistently seen in Region D. © 1968.
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页码：1213 / &
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共 23 条
[11]   CHARACTERIZATION OF SALIVARY COMPONENTS SEPARATED BY PAPER ELECTROPHORESIS [J].
MANDEL, ID ;
ELLISON, SA .
ARCHIVES OF ORAL BIOLOGY, 1961, 3 (02) :77-+
[12]   ZONE ELECTROPHORESIS OF HUMAN PAROTID SALIVA IN ACRYLAMIDE GEL [J].
MEYER, TS ;
LAMBERTS, BL .
NATURE, 1965, 205 (4977) :1215-&
[13]  
NEIDLE A, 1964, SCIENCE, V145, P1058
[14]   ELECTROPHORETIC AND ULTRACENTRIFUGAL COMPONENTS OF HUMAN SALIVARY SECRETIONS [J].
PATTON, JR ;
PIGMAN, W .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1959, 81 (12) :3035-3039
[15]   AMYLASE IN ELECTROPHORETIC AND ULTRACENTRIFUGAL PATTERNS OF HUMAN PAROTID SALIVA [J].
PATTON, JR ;
PIGMAN, W .
SCIENCE, 1957, 125 (3261) :1292-1293
[16]  
PEARSE AGE, 1960, HISTOCHEMISTRY T8EOR, P259
[17]   DISK ELECTROPHORESIS OF BASIC PROTEINS AND PEPTIDES ON POLYACRYLAMIDE GELS [J].
REISFELD, RA ;
LEWIS, UJ ;
WILLIAMS, DE .
NATURE, 1962, 195 (4838) :281-&
[18]  
SCHNEYER LH, 1967, SECRETORY MECHANI ED
[19]  
SHEAR M, 1964, EXPTL CELL RES, V32, P174
[20]  
WEINSTEIN EPHRAIM, 1960, JOUR DENTAL RES, V39, P110, DOI 10.1177/00220345600390010401
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