Mayflies from 80 sites across Connecticut were studied. A list of 109 species, including 34 new State records, was compiled. Two-way Indicator Species Analysis (TSA) was used to investigate block structure in the overall species data matrix. Site classes produced at successive division levels in repeated TSA runs showed no discernible stable geographic trends. Similarities among sites within a class and variation between classes resulted from differences in attributes of aquatic habitat structure that are not constrained within the context of ecoregions/subregions proposed for Connecticut.