CHARACTERIZATION OF MONOCLONAL-ANTIBODIES AGAINST HUMAN-LEUKOCYTE 5-LIPOXYGENASE

被引：1
作者：
ETHIER, D
EVANS, JF
机构：
[1] Department of Pharmacology, Merck Frosst Centre for Therapeutic Research, Pointe Claire-Dorval, Que. H9R 4P8
来源：
PROSTAGLANDINS LEUKOTRIENES AND ESSENTIAL FATTY ACIDS | 1992年 / 45卷 / 04期
关键词：
D O I：
10.1016/0952-3278(92)90081-S
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Four mouse monoclonal IgG1 antibody-producing cell lines (5LO-1, 5LO-2, 5LO-3, 5LO-4), produced against highly purified human leukocyte 5-lipoxygenase have been characterized. The monoclonal antibodies produced by these cell lines exhibited differential reactivity against 5-lipoxygenase as determined by ELISA and immunoprecipitation analyses. Monoclonal antibodies 5LO-2 and 5LO-3 inhibited the activity of recombinant human leukocyte 5-lipoxygenase in a dose-dependent manner. This inhibition was selective for 5-lipoxygenase activity since these monoclonal antibodies did not inhibit human leukocyte 15-lipoxygenase or porcine leukocyte 12-lipoxygenase.
引用
收藏
页码：259 / 265
页数：7
相关论文
共 50 条
  • [31] CHARACTERIZATION OF MONOCLONAL-ANTIBODIES AGAINST HUMAN ROTAVIRUS HEMAGGLUTININ
    KITAOKA, S
    FUKUHARA, N
    TAZAWA, F
    SUZUKI, H
    SATO, T
    KONNO, T
    EBINA, T
    ISHIDA, N
    JOURNAL OF MEDICAL VIROLOGY, 1986, 19 (04) : 313 - 323
  • [32] PRODUCTION AND CHARACTERIZATION OF MONOCLONAL-ANTIBODIES AGAINST HUMAN GLYCOALBUMIN
    COHEN, MP
    HUD, E
    JOURNAL OF IMMUNOLOGICAL METHODS, 1989, 117 (01) : 121 - 129
  • [33] PRODUCTION AND CHARACTERIZATION OF MONOCLONAL-ANTIBODIES AGAINST HUMAN APOLIPOPROTEINS
    BREEN, D
    OKENNEDY, R
    HALLINAN, F
    BIOCHEMICAL SOCIETY TRANSACTIONS, 1985, 13 (02) : 478 - 478
  • [34] GENERATION AND CHARACTERIZATION OF MONOCLONAL-ANTIBODIES AGAINST HUMAN RANTES
    LU, J
    LI, B
    SCHALL, TJ
    KIM, KJ
    JOURNAL OF CELLULAR BIOCHEMISTRY, 1994, : 286 - 286
  • [35] INDUCTION OF HUMAN-COMPLEMENT ACTIVATION WITHOUT CYTOLYSIS BY MOUSE MONOCLONAL-ANTIBODIES TO HUMAN-LEUKOCYTE ANTIGENS
    SUGITA, K
    MAJDIC, O
    STOCKINGER, H
    HOLTER, W
    BURGER, R
    EGUCHI, M
    FURUKAWA, T
    KNAPP, W
    EXPERIMENTAL HEMATOLOGY, 1987, 15 (05) : 486 - 486
  • [36] REGULATION OF THE HUMAN-LEUKOCYTE 5-LIPOXYGENASE - STIMULATION BY MICROMOLAR CA-2+ LEVELS AND PHOSPHATIDYLCHOLINE VESICLES
    PUUSTINEN, T
    SCHEFFER, MM
    SAMUELSSON, B
    BIOCHIMICA ET BIOPHYSICA ACTA, 1988, 960 (03) : 261 - 267
  • [37] INDUCTION OF HUMAN-COMPLEMENT ACTIVATION WITHOUT CYTOLYSIS BY MOUSE MONOCLONAL-ANTIBODIES TO HUMAN-LEUKOCYTE ANTIGENS
    SUGITA, K
    MAJDIC, O
    STOCKINGER, H
    HOLTER, W
    BURGER, R
    KNAPP, W
    TRANSPLANTATION, 1987, 43 (04) : 570 - 574
  • [38] MONOCLONAL-ANTIBODIES NEUTRALIZING HUMAN-LEUKOCYTE ACID-LABILE AND THERMOLABILE INTERFERON-ALPHA
    KONTSEK, P
    BORECKY, L
    LACKOVIC, V
    KONTSEKOVA, E
    SUJANOVA, Z
    NOVAK, M
    ACTA VIROLOGICA, 1989, 33 (03) : 254 - 261
  • [39] CHARACTERIZATION OF THE HUMAN 5-LIPOXYGENASE GENE
    FUNK, CD
    MATSUMOTO, T
    HOSHIKO, S
    RADMARK, O
    SAMUELSSON, B
    ADVANCES IN PROSTAGLANDIN THROMBOXANE AND LEUKOTRIENE RESEARCH, 1989, 19 : 470 - 473
  • [40] RECOGNITION OF BINDING-SITES ON CANDIDA-ALBICANS BY MONOCLONAL-ANTIBODIES TO HUMAN-LEUKOCYTE ANTIGENS
    MAYER, CL
    DIAMOND, RD
    EDWARDS, JE
    INFECTION AND IMMUNITY, 1990, 58 (11) : 3765 - 3769
12345