CHARACTERIZATION OF MONOCLONAL-ANTIBODIES AGAINST HUMAN-LEUKOCYTE 5-LIPOXYGENASE

被引：1
|
作者：
ETHIER, D
EVANS, JF
机构：
[1] Department of Pharmacology, Merck Frosst Centre for Therapeutic Research, Pointe Claire-Dorval, Que. H9R 4P8
来源：
PROSTAGLANDINS LEUKOTRIENES AND ESSENTIAL FATTY ACIDS | 1992年 / 45卷 / 04期
关键词：
D O I：
10.1016/0952-3278(92)90081-S
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Four mouse monoclonal IgG1 antibody-producing cell lines (5LO-1, 5LO-2, 5LO-3, 5LO-4), produced against highly purified human leukocyte 5-lipoxygenase have been characterized. The monoclonal antibodies produced by these cell lines exhibited differential reactivity against 5-lipoxygenase as determined by ELISA and immunoprecipitation analyses. Monoclonal antibodies 5LO-2 and 5LO-3 inhibited the activity of recombinant human leukocyte 5-lipoxygenase in a dose-dependent manner. This inhibition was selective for 5-lipoxygenase activity since these monoclonal antibodies did not inhibit human leukocyte 15-lipoxygenase or porcine leukocyte 12-lipoxygenase.
引用
收藏
页码：259 / 265
页数：7
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