Molecularly Imprinted Quartz Crystal Microbalance Sensor (QCM) for Bilirubin Detection

被引：34

作者：

Cicek, Cigdem ^{[1
]}

Yilmaz, Fatma ^{[2
]}

Ozgur, Erdogan ^{[1
]}

Yavuz, Handan ^{[1
]}

Denizli, Adil ^{[1
]}

机构：

[1] Hacettepe Univ, Dept Chem, TR-06800 Ankara, Turkey

[2] Abant Izzet Baysal Univ, Dept Chem Technol, TR-14900 Bolu, Turkey

来源：

CHEMOSENSORS | 2016年 / 4卷 / 04期

关键词：

molecular imprinting technique; bilirubin; QCM; PHEMA; nanosensor;

D O I：

10.3390/chemosensors4040021

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

This study aims the preparation of a QCM sensor for the detection of bilirubin in human plasma. Bilirubin-imprinted poly-(2-hydroxyethyl methacrylate-N-methacryloyl-l-tryptophan methyl ester) (PHEMATrp) nanofilm (MIP) on the gold surface of a QCM chip was synthesized by the molecular imprinting technique. Meanwhile, the non-imprinted PHEMATrp (NIP) nanofilm was synthesized by the same experimental technique to examine the imprinting effect. Characterization of MIP and NIP nanofilms on the QCM chip surface was achieved by atomic force microscopy (AFM), ellipsometry, Fourier transform infrared spectrophotometry-attenuated total reflectance (FTIR-ATR) and contact angle measurements (CA). The observations indicated that the nanofilm was almost in a monolayer. Thereinafter, the imprinted and the non-imprinted QCM chips were connected to the QCM system to investigate kinetic and affinity properties. In order to examine the selectivity of the MIP-PHEMATrp nanofilm, competitive adsorption of bilirubin with cholesterol and estradiol was performed. Limit of detection (LOD) and limit of quantitation (LOQ) values were calculated as 0.45 g/mL and 0.9 g/mL, respectively.

引用

页数：13

共 45 条

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