ALTERNATIVE SPLICING GENERATES 2 ISOFORMS OF THE ALPHA-2 SUBUNIT OF THE INHIBITORY GLYCINE RECEPTOR

被引：117
作者：
KUHSE, J
KURYATOV, A
MAULET, Y
MALOSIO, ML
SCHMIEDEN, V
BETZ, H
机构：
[1] MAX PLANCK INST BRAIN RES,DEUTSCHORDENSTR 46,W-6000 FRANKFURT,GERMANY
[2] UNIV HEIDELBERG,ZENTRUM MOLEK BIOL HEIDELBERG,W-6900 HEIDELBERG,GERMANY
来源：
FEBS LETTERS | 1991年 / 283卷 / 01期
关键词：
GLYCINE RECEPTOR; ALTERNATIVE SPLICING; RECEPTOR HETEROGENEITY; BRAIN DEVELOPMENT;
D O I：
10.1016/0014-5793(91)80557-J
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The inhibitory glycine receptor (GlyR) is a ligand-gated chloride channel protein which displays developmental heterogeneity in the mammalian central nervous system. Here we describe 2 novel cDNA variants of the rat GlyR alpha-2 subunit and demonstrate that alternative splicing generates these 2 isoforms. The deduced protein sequences (alpha-2A and alpha-2B) exhibit 99% identity with the previously characterized human alpha-2 subunit. In situ hybridization revealed expression of both alpha-2A and alpha-2B mRNAs in the prenatal rat brain, suggesting that these variant proteins may have a role in synaptogenesis. Heterologous expression in Xenopus oocytes showed that the more abundantly expressed alpha-2A subunit forms strychnine-sensitive ion channels which resemble human alpha-2 subunit GlyRs in their electrophysiological properties.
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页码：73 / 77
页数：5
相关论文
共 22 条
[1]  
Aprison MH, 1978, ADV NEUROCHEM, V3, P203
[2]   GLYCINE RECEPTOR HETEROGENEITY IN RAT SPINAL-CORD DURING POSTNATAL-DEVELOPMENT [J].
BECKER, CM ;
HOCH, W ;
BETZ, H .
EMBO JOURNAL, 1988, 7 (12) :3717-3726
[3]   THE HUMAN MUSCLE NICOTINIC ACETYLCHOLINE-RECEPTOR ALPHA-SUBUNIT EXISTS AS 2 ISOFORMS - A NOVEL EXON [J].
BEESON, D ;
MORRIS, A ;
VINCENT, A ;
NEWSOMDAVIS, J .
EMBO JOURNAL, 1990, 9 (07) :2101-2106
[4]   THE MAMMALIAN GLYCINE RECEPTOR - BIOLOGY AND STRUCTURE OF A NEURONAL CHLORIDE CHANNEL PROTEIN [J].
BETZ, H ;
BECKER, CM .
NEUROCHEMISTRY INTERNATIONAL, 1988, 13 (02) :137-146
[5]   A METHOD FOR ISOLATION OF INTACT, TRANSLATIONALLY ACTIVE RIBONUCLEIC-ACID [J].
CATHALA, G ;
SAVOURET, JF ;
MENDEZ, B ;
WEST, BL ;
KARIN, M ;
MARTIAL, JA ;
BAXTER, JD .
DNA-A JOURNAL OF MOLECULAR & CELLULAR BIOLOGY, 1983, 2 (04) :329-335
[6]   COORDINATE REGULATION OF RNAS ENCODING 2 ISOFORMS OF THE RAT MUSCLE NICOTINIC ACETYLCHOLINE-RECEPTOR BETA-SUBUNIT [J].
GOLDMAN, D ;
TAMAI, K .
NUCLEIC ACIDS RESEARCH, 1989, 17 (08) :3049-3056
[7]   MEMBERS OF A NICOTINIC ACETYLCHOLINE-RECEPTOR GENE FAMILY ARE EXPRESSED IN DIFFERENT REGIONS OF THE MAMMALIAN CENTRAL-NERVOUS-SYSTEM [J].
GOLDMAN, D ;
DENERIS, E ;
LUYTEN, W ;
KOCHHAR, A ;
PATRICK, J ;
HEINEMANN, S .
CELL, 1987, 48 (06) :965-973
[8]   PURIFICATION AND CHARACTERIZATION OF THE GLYCINE RECEPTOR OF PIG SPINAL-CORD [J].
GRAHAM, D ;
PFEIFFER, F ;
SIMLER, R ;
BETZ, H .
BIOCHEMISTRY, 1985, 24 (04) :990-994
[9]   CLONING AND EXPRESSION OF THE 58 KD-BETA SUBUNIT OF THE INHIBITORY GLYCINE RECEPTOR [J].
GRENNINGLOH, G ;
PRIBILLA, I ;
PRIOR, P ;
MULTHAUP, G ;
BEYREUTHER, K ;
TALEB, O ;
BETZ, H .
NEURON, 1990, 4 (06) :963-970
[10]   THE STRYCHNINE-BINDING SUBUNIT OF THE GLYCINE RECEPTOR SHOWS HOMOLOGY WITH NICOTINIC ACETYLCHOLINE-RECEPTORS [J].
GRENNINGLOH, G ;
RIENITZ, A ;
SCHMITT, B ;
METHFESSEL, C ;
ZENSEN, M ;
BEYREUTHER, K ;
GUNDELFINGER, ED ;
BETZ, H .
NATURE, 1987, 328 (6127) :215-220
123