REGIONAL LOCALIZATION OF 56 NEW HUMAN CHROMOSOME-18 SPECIFIC YEAST ARTIFICIAL CHROMOSOMES

被引：5
作者：
CHANG, E
LUNA, J
GIACALONE, J
UYAR, D
SILVERMAN, GA
FRANCKE, U
机构：
[1] STANFORD UNIV, MED CTR, SCH MED, DEPT GENET, STANFORD, CA 94305 USA
[2] STANFORD UNIV, MED CTR, SCH MED, HOWARD HUGHES MED INST, STANFORD, CA 94305 USA
[3] HARVARD UNIV, SCH MED, DEPT PEDIAT, JOINT PROGRAM NEONATOL, BOSTON, MA 02115 USA
来源：
CYTOGENETICS AND CELL GENETICS | 1994年 / 65卷 / 1-2期
关键词：
D O I：
10.1159/000133620
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Fifty-six chromosome 18-specific yeast artificial chromosomes (YAC) were isolated from a human monochromosomal somatic cell hybrid DNA library. Fluorescence in situ hybridization revealed that the clones were evenly distributed throughout the chromosome. The clones, with an average insert size of 250 kb, cover about 18% of the euchromatic part of chromosome 18. Of 90 STS markers tested, 17 were represented in this YAC collection. Together with our previously reported set of unique chromosome 18-specific YACs, we now have available 111 regionally mapped, essentially nonchimeric, clones that provide more than 30% coverage and form a framework for the complete physical map.
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页码：136 / 139
页数：4
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