PARTIAL-PURIFICATION OF PLASMA PHENOLOXIDASE OF THE YELLOW-FEVER MOSQUITO AEDES-AEGYPTI L (DIPTERA, CULICIDAE)

被引：11

作者：

BURKS, CS ^{[1
]}

FUCHS, MS ^{[1
]}

机构：

[1] UNIV NOTRE DAME,DEPT BIOL SCI,NOTRE DAME,IN 46556

来源：

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY | 1995年 / 110卷 / 03期

关键词：

AEDES AEGYPTI; MOSQUITO; PLASMA PHENOLOXIDASE; TYROSINASE; DETECTION METHOD; PROTEIN PURIFICATION;

D O I：

10.1016/0305-0491(94)00166-R

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A nitrocellulose-based assay was de,eloped using a dot-blot apparatus to detect phenoloxidase activity in column fractions. Using this assay, plasma phenoloxidase was partially purified from Aedes aegypti larvae using hydrophobic interaction chromatography, gel filtration, and ion-exchange chromatography. The molecular weight (M(r)) native enzyme was 130,000, and it contained subunits of 76,000, 62,000, and 58,000. Two phenoloxidase peaks were observed by ion exchange chromatography, and these Fractions had distinct polypeptide profiles as detected by SDS-PAGE.

引用

页码：641 / 647

页数：7

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