INHIBITION BY AGMATINE OF PROTEASES PRODUCED BY MARINE-BACTERIA

被引：0

作者：

MORITA, J ^{[1
]}

ODAGAMI, T ^{[1
]}

TAKAMA, K ^{[1
]}

SUZUKI, S ^{[1
]}

机构：

[1] HOKKAIDO UNIV,FAC FISHERIES,DEPT FOOD SCI & TECHNOL,HAKODATE,HOKKAIDO 041,JAPAN

来源：

MICROBIOS | 1993年 / 74卷 / 301期

关键词：

AGMATINE; EXTRACELLULAR PROTEASES; MARINE BACTERIA;

D O I：

暂无

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Naturally occurring amino acids and diamines such as arginine, agmatine and putrescine, inhibited proteases produced by the marine putrefactive bacteria Shewanella putrefaciens S29 and Alteromonas haloplanktis S5B, although the compounds did not inhibit bacterial growth. Among the amino acids and diamines tested, agmatine showed the greatest inhibitory effect, that is, 1 mM of agmatine inhibited 60% of the activity of the S. putrefaciens enzyme and 80% of the A. haloplanktis protease. Kinetic data indicated apparent competitive inhibition with the substrate (Bz-Arg-MCA) in the protease assay.

引用

页码：205 / 209

页数：5

共 16 条

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