SEQUENCE OF THE VANC GENE OF ENTEROCOCCUS-GALLINARUM BM4174 ENCODING A D-ALANINE - D-ALANINE LIGASE-RELATED PROTEIN NECESSARY FOR VANCOMYCIN RESISTANCE

被引：131
作者：
DUTKAMALEN, S
MOLINAS, C
ARTHUR, M
COURVALIN, P
机构：
[1] Unité des Agents Antibactériens, Institut Pasteur
来源：
GENE | 1992年 / 112卷 / 01期
关键词：
ANTIBIOTIC RESISTANCE; CELL WALL SYNTHESIS; AMPLIFICATION; GLYCOPEPTIDE; RECOMBINANT DNA; INSERTIONAL INACTIVATION;
D O I：
10.1016/0378-1119(92)90302-6
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
The amplification product obtained with DNA from vancomycin-resistant (Vm(R)) Enterococcus gallinarum BM4174 and a pair of degenerate oligodeoxyribonucleotides that correspond to conserved amino acid (aa) motifs in Escherichia coli D-alanine (D-Ala):D-Ala ligases and in En. faecium Vm(R) protein (VanA) was used as a probe to clone the vanC gene of that strain. The vanC product, with a calculated M(r) of 37504, exhibits 29 to 38% aa identity with VanA and E. coli ligases. Insertional inactivation of vanC led to Vm sensitivity of BM4174 suggesting that the gene may encode a D-Ala:D-Ala ligase of altered specificity.
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收藏
页码：53 / 58
页数：6
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