TRANSFORMATION OF BACILLUS-SUBTILIS BY ELECTROPORATION

被引：11
|
作者：
STEPHENSON, M [1 ]
JARRETT, P [1 ]
机构：
[1] INST HORT RES,LITTLEHAMPTON BN17 6LP,W SUSSEX,ENGLAND
来源：
BIOTECHNOLOGY TECHNIQUES | 1991年 / 5卷 / 01期
关键词：
D O I：
10.1007/BF00152746
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Optimal conditions for the transformation of Bacillus subtilis by electroporation were achieved. Frequencies of greater than 10(5) transformants/mu-g of plasmid DNA were obtained for a number of strains and plasmids. Increased transformation efficiency of mini-prep DNA from B. subtilis and Escherichia coli was obtained after microdialysis.
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页码：9 / 12
页数：4
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