INCREASED TRANSCRIPTION AND TRANSLATION OF HEME OXYGENASE IN CHINESE-HAMSTER FIBROBLASTS FOLLOWING PHOTODYNAMIC STRESS OR PHOTOFRIN-II INCUBATION

被引：83

作者：

GOMER, CJ

LUNA, M

FERRARIO, A

RUCKER, N

机构：

[1] UNIV SO CALIF,SCH MED,DEPT PEDIAT,LOS ANGELES,CA 90027

[2] UNIV SO CALIF,SCH MED,DEPT RADIAT ONCOL,LOS ANGELES,CA 90027

来源：

PHOTOCHEMISTRY AND PHOTOBIOLOGY | 1991年 / 53卷 / 02期

关键词：

D O I：

10.1111/j.1751-1097.1991.tb03934.x

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Porphyrin mediated photosensitization can enhance the transcription and translation of several oxidative stress genes. In this study, we report on the enhanced expression of the gene encoding for heme oxygenase in Chinese hamster fibroblasts by; (1) incubation in Photofrin II; (2) Photofrin II mediated photosensitization; and (3) photosensitization induced by Rose Bengal. Increased expression of heme oxygenase mRNA was accompanied by a concomitant increase in the synthesis of the 34 kDa heme oxygenase protein. Western blot analysis using antibody to heme oxygenase confirmed the immunoreactivity of the 34 kDa protein induced by Photofrin II and PDT. These results demonstrate that heme oxygenase can be activated by non-metalloporphyrins as well as by photosensitization associated with singlet oxygen mediated subcellular injury.

引用

页码：275 / 279

页数：5

共 24 条

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